ABSTRACT
To establish a method for studying fingerprint of Shengxuening tablets. With chlorin e6 as the reference substance, SHISEIDO Capcell-pak C18 (4.6 mm x 250 mm, 5 microm) analytical column was adopted and eluted with 0.2% formic acid ( containing 20 mmol x L(-1) TBAB) (A) and acetonitrile-methanol-acetone (50: 50: 5) (B). The detection wavelength was set 392 nm. The volume flow rate was 1.0 mL x min(-1). The temperature of column was 45 degrees C. Totally 10 common peaks were indicated on the HPLC fingerprint, with RSDs for variable retention values in common peaks below 0.50%. UPLC/DAD/Q-TOF-MS xevo G2 Q-TOF LC/MS was adopted to preliminarily indentify six chromatographic peaks. The main ingredient in Shengxuening tablets was ferrous derivative, which was mainly composed of Fe chlorin p6, Fe chlorin e6 and Fe isochlorin e4.
Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Quality Control , TabletsABSTRACT
Objective: To establish a human hepatocellular carcinoma cell line derived from portal vein tumor thrombus and study its biological characteristics. Methods: Specimens of liver cancer and its metastasis-portal vein tumor thrombi (PVTT) were freshly resected during operation. The tissues were primary cultured by collagenase I digestion method and proliferated. The cell line derived from PVTT grew well. The biological characteristics of the cells were studied by light microscopy, electron microscopy, chromosome analysis, and transplantation experiment. Results: The cell line derived from portal vein tumor thrombus, named CSQT-1, has been cultivated for one year and sub-cultured for more than 100 passages in vitro. The cells either grew as compact colonies (in clusters) or as a monolayered sheet, with a doubling time of 48 h, and exhibited a typical malignant epithelial morphology. The median range of chromosome number was 87-90. Tumor nodes were observed under the skin of nude mice after subcutaneous xenograft transplantation for a month. The established cell line stably expressed CD133. Conclusion: We have successfully established a human hepatocellular carcinoma cell line from PVTT.